论文部分内容阅读
目的:探讨高尿酸大鼠血尿酸与血管内皮功能的关系。方法:雄性SD大鼠36只,随机均分为正常对照组、模型组和别嘌醇治疗组。使用高酵母膏饲料联合氧嗪酸钾腹腔注射6周诱导大鼠高尿酸模型。别嘌醇治疗组除给予造模剂外同时以别嘌醇灌胃6周。定期测量大鼠收缩压。6周后处死大鼠,检测各组血清尿酸(SUA)、一氧化氮(NO)、内皮素-1(ET-1)、收缩压(SBP)等的变化,用免疫组化法检测大鼠主动脉内膜层内皮型一氧化氮合酶(eNOS)的表达量。结果:与正常对照组相比,模型组大鼠SUA水平[(45.1±5.6)μmol/L∶(216.0±6.2)μmol/L]显著升高(P<0.001),ET-1[(85.4±8.7)μg/L∶(163.1±7.2)μg/L]、SBP[(115.7±4.1)mm-Hg∶(156.0±3.8)mmHg]显著升高,血NO[(24.1±2.0)μmol/L∶(17.2±3.4)μmol/L]及主动脉内膜层eNOS[(48.3±4.2)∶(38.3±4.5)]表达量显著降低(P均<0.05);与模型组比较,别嘌醇组SUA[(44.8±4.3)μmol/L]、ET-1[(92.8±5.0)μg/L]、SBP[(119.2±4.3)mmHg]水平显著降低,血NO[(22.1±2.2)μmol/L]和主动脉内膜eNOS的表达量(46.1±4.2)明显升高(P均<0.05),别嘌醇组与正常对照组比较上述指标无明显差异(P>0.05)。SUA与SBP、ET-1呈正相关(r=0.98、0.98,P均<0.001),与NO呈负相关(-0.70,P<0.001)。结论:高尿酸血症与大鼠血管内皮功能紊乱密切相关。血一氧化氮的降低和内皮素-1的升高可能是发生高血压的重要原因之一。别嘌醇具有保护血管内皮功能的作用。
Objective: To investigate the relationship between serum uric acid and vascular endothelial function in rats with hyperuricemia. Methods: Thirty-six male SD rats were randomly divided into normal control group, model group and allopurinol treatment group. The rat model of hyperuricemia was induced by intraperitoneal injection of high yeast extract and oxonic acid potassium for 6 weeks. The allopurinol group was given gavage for 6 weeks besides allopurinol. Systolic pressure was measured regularly. After 6 weeks, the rats were sacrificed and the changes of serum SUA, ET, ET-1 and SBP were measured. The expressions of superoxide dismutase The expression of endothelial nitric oxide synthase (eNOS) in the aortic intima. Results: Compared with normal control group, the level of SUA in model group was significantly higher than that in control group [(45.1 ± 5.6) μmol / L vs (216.0 ± 6.2) μmol / L vs (24.7 ± 2.0) μmol / L) was significantly higher than that of SBP [(115.7 ± 4.1) mmHg: (156.0 ± 3.8) mmHg] (17.2 ± 3.4) μmol / L and eNOS in aortic endocardium [(48.3 ± 4.2) vs (38.3 ± 4.5)] (P all <0.05). Compared with the model group, [(44.8 ± 4.3) μmol / L], ET-1 [(92.8 ± 5.0) μg / L] and SBP [(119.2 ± 4.3) (46.1 ± 4.2) (P <0.05). There was no significant difference between the allopurinol group and the normal control group (P> 0.05). There was a positive correlation between SUA and SBP and ET-1 (r = 0.98, 0.98, P <0.001) and negatively correlated with NO (-0.70, P <0.001). Conclusion: Hyperuricemia is closely related to vascular endothelial dysfunction in rats. Reduced blood nitric oxide and elevated endothelin-1 may be one of the important causes of hypertension. Allopurinol has the function of protecting vascular endothelial function.