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目的 应用质子磁共振波谱 (1H- MRS) 技术, 进行慢性帕金森病 (PD) 1 甲基 4 苯基 1, 2, 3, 6- 四氢吡啶 (MPTP) 猴模型自体颈动脉体 (CB) 细胞纹状体区移植术后细胞结构生存能力检测。方法 采用 4 只按非人类灵长类帕金森病评分量表 (PPRS) 评价, 行为学症状持续稳定 1 个月的慢性 PD MPTP雄性猴模型, 应用 GE Signa1 5T NV/i磁共振扫描系统进行移植术前定位, 取一侧自体颈动脉体, 使用 Leksell立体导向系统行纹状体区立体定向移植术, 于移植术后 8 周内每 2 周行双侧纹状体区1H MRS 采集, 检测大脑主要代谢物氮 乙酰天门冬氨酸(NAA)、胆碱化合物 (Cho)、肌酸及磷酸肌酸 (Cr), 采用随机软件FuncTool 4 .0对各代谢物含量行半定量分析, 观察NAA/Cr和Cho/Cr的变化, 以配对 t检验行统计学分析, 按PPRS标准进行移植后行为学评分并与代谢物比值进行对照分析。结果 移植术4周后, 移植侧纹状体区 NAA/Cr逐渐增高, Cho/Cr逐渐下降, 术后 6 周与未移植侧纹状体区比较出现显著性差异 (P<0 .05)。行为学评分呈下降趋势。结论 1H MRS能够显示慢性PD MPTP猴模型移植区域的代谢及生化改变, 自体CB细胞移植能够促进局部多巴胺神经元的生长, 可以作为有效手段应用于 PD的治疗。
Objective To investigate the effects of carotid body (CB) in chronic Parkinson’s disease (PD) 1 methyl-4-phenyl-1,2,4,6-tetrahydropyridine (MPTP) monkey model by proton magnetic resonance spectroscopy (1H- Detection of viability of cell structure after transplantation in. Methods Four chronic PD MPTP male monkeys evaluated by non-human primate Parkinson’s Disease Rating Scale (PPRS) and behavioral symptoms were stable for 1 month were enrolled in the study. They were implanted with GE Signa1 5T NV / i magnetic resonance imaging system Preoperative localization of the carotid body on one side, using Leksell Stereotactic Stereotactic Graft Stereotactic Graft stereotactic transplantation within 8 weeks after transplantation every 2 weeks line 1H MRS acquisition of the striatum to detect the main brain metabolism NAA, Cho, creatine and creatine phosphate (Cr) were assayed by semi-quantitative FuncTool 4.0. The contents of NAA / Cr and Cho / Cr changes, paired t test line statistical analysis, PPRS standards after transplantation behavioral score and the ratio of metabolites were compared. Results After 4 weeks of transplantation, NAA / Cr increased gradually and the ratio of Cho / Cr decreased gradually in the grafted area. There was a significant difference (P <0. 05) between the 6 weeks after transplantation and the non-grafted area. Behavioral score showed a downward trend. Conclusion 1H MRS can show the metabolism and biochemical changes in transplantation area of chronic PD MPTP monkey model. Autologous CB cell transplantation can promote the growth of local dopamine neurons and can be used as an effective method in the treatment of PD.