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为明确海南省近几年发生的一品红细菌性叶部病害的病原菌特别是该病原菌的分子特征,为该病害的治理提供可行的依据,本文描述了2005年海南省部分花圃一品红植株上发生的细菌性叶斑病症状,并通过致病性测定、BIOLOG分析和16S rDNA序列比较将分离的病原菌鉴定为黄单胞菌属(Xanthomonas);部分碳源利用测定进一步显示2005年海南分离的菌株与2004年海南报道的细菌性疫病病原菌存在差异,但与杭州地区报道的一品红细菌性叶斑病菌基本一致。利用黄单胞菌模式种典型菌株的核糖体DNA内转录间隔区(internal transcribed spacer,ITS)序列构建系统发育树,结果显示3个海南菌株与巴豆黄单胞菌(Xanthomonas codiaei)和地毯草黄单胞菌(X.axonopodis)单独聚合成群,其中与巴豆黄单胞菌亲缘关系最近。
In order to clarify the pathogens of the bacterial leaf disease of poinsettia in recent years in Hainan Province, especially the molecular characteristics of the pathogen, this paper provided a feasible basis for the treatment of this disease. This article described the bacteria that occurred on some flowerpot poinsettia plants in Hainan Province in 2005 And the pathogen was identified. The pathogen was identified by pathological analysis, BIOLOG analysis and 16S rDNA sequence analysis. The results showed that the isolates were identified as Xanthomonas. The pathogen of bacterial blight reported in Hainan was different in recent years, but it was basically the same as that of the P. ergenicus reported in Hangzhou. Phylogenetic tree was constructed using ribosomal DNA internal transcribed spacer (ITS) sequences from Xanthomonas campestris species. The results showed that the three Hainan isolates belonged to Xanthomonas codiaei, X. axonopodis aggregates alone, with the closest relationship with Xanthomonas campestris.