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目的探讨重组内皮抑素(Endostatin)对兔角膜新生血管(corneal neovascularization,CNV)形成的影响。方法新西兰白兔18只,随机分为三组,每组6只12眼,采用角膜缝线法制备兔CNV模型,其中两组分别以浓度为10 μg/ml、50μg/ml重组内皮抑素点眼,4次/d,连续19 d,并以生理盐水点眼作对照。CNV生长面积采用计算机图像分析系统进行分析比较。结果10μg/ml、50μg/ml重组内皮抑素实验组自第7d开始CNV平均生长面积明显低于对照组(P<0.05),两实验组CNV面积比较,差异无显著意义(P>0.05)。角膜组织病理学检查,实验组新生血管明显稀少,实验组与对照组炎性细胞成分及浸润程度相似。结论浓度10 μg/ml、50μg/ml重组内皮抑素具有明显抑制角膜新生血管生长的作用,而无毒副作用,为临床防治CNV提供了一种新的途径.
Objective To investigate the effect of recombinant endostatin on the formation of corneal neovascularization (CNV) in rabbits. Methods Eighteen New Zealand white rabbits were randomly divided into three groups, with 6 eyes in each group. Rabbit CNV model was established by corneal suture method. Rabbits with CNV were treated with 10 μg / ml and 50 μg / ml recombinant endostatin , 4 times / d, for 19 days, with saline as control. CNV growth area using computer image analysis system for analysis and comparison. Results The mean area of CNV growth in 10μg / ml and 50μg / ml recombinant endostatin group was significantly lower than that in control group from the 7th day (P <0.05). There was no significant difference in CNV area between the two experimental groups (P> 0.05). Corneal histopathological examination, experimental group of new blood vessels was significantly less, experimental group and control group of inflammatory cell composition and degree of infiltration was similar. Conclusions Recombinant human endostatin (10 μg / ml, 50 μg / ml) can inhibit the growth of corneal neovasculature without any toxic and side effects, which provides a new approach for clinical prevention and treatment of CNV.