对柑橘原生质体的超低温保存进行研究 ,发现玻璃化溶液加入小牛血清蛋白对原生质体的超低温保存有利 ,不同品种的胚性愈伤组织原生质体超低温保存后的成活率不同 ;不同倍性的伏令夏橙原生质体超低温保存后的成活率也不同 ,其中 2倍体成活率最高 ,其次为 4倍体 ,6倍体成活率最低 .超低温保存后的原生质体在BH3培养基中能够恢复分裂 ,形成细胞团 Cryopreservation of citrus protoplasts was studied and it was found that the addition of bovine serum albumin to the cryopreservation of protoplasts was beneficial. The survival rates of embryogenic callus protoplasts of different varieties after cryopreservation were different. The survival rate of summer orange protoplasts after cryopreservation was also different, in which the diploid survival rate was the highest, followed by the tetraploid, and the lowest in the 6-ploid survival rate.The cryopreservation protoplasts were able to restore the division in BH3 medium, Formation of cell clusters