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目的:研究肺保护性通气策略联合山莨宕碱在急性肺损伤(ALI)动物模型中的肺保护作用。方法:将40只雌性wistar大白鼠经尾静脉注射油酸制成急性肺损伤模型,随机分成5组(每组8只):A组为对照组;B组为山莨宕碱治疗组(5mg·kg-1·h-1);C组采用大潮气量(15ml·kg-1)进行机械通气;D组采用小潮气量(5ml·kg-1)通气;E组为小潮气量机械通气联合山莨宕碱。分别监测各组治疗前后各时间点的动脉血气和mPAP;治疗4h后,处死大白鼠,取出肺组织,制片后在光镜下观察肺损伤程度。结果:与治疗前相比,A组氧合指数(OI)进行性下降(P<0.05);B组OI呈上升趋势(第3,4h点上升较为明显);D,E两组OI各时点也呈上升趋势,且各时点有显著性差异;C组OI呈先上升(1,2h明显高于其他组)后大幅度下降(在3,4h显著低于其他组)的趋势;E组在3,4h显著高于其他组。A组mPAP呈上升趋势,各时点均显著高于治疗前;B,D,E组呈下降趋势,在2,3,4h点均低于A组;C组前两时点显著低于治疗前和其他各组。C组的肺损伤积分均高于其他组(P(0.05),E组最低,B和D组得的肺损伤积分显著低于A组。结论:保护性肺通气策略联合山莨宕碱对实验性ALI动物模型具有协同治疗效果。
Objective: To study the lung protection effect of pulmonary protective ventilation strategy combined with anisodamine in acute lung injury (ALI) animal model. Methods: A total of 40 female Wistar rats were injected with oleic acid through the tail vein to establish acute lung injury model. They were randomly divided into 5 groups (8 in each group): group A was control group; group B was treated with anisodamine · Kg-1 · h-1). In group C, mechanical ventilation was performed with large tidal volume (15ml · kg-1), group D with low tidal volume (5ml · kg-1) ventilation and group E with small tidal volume ventilation Damp alkali. The arterial blood gas and mPAP were monitored before and after treatment at each time point. After 4 hours of treatment, the rats were sacrificed and the lung tissue was taken out. The degree of lung injury was observed under light microscope after preparation. Results: Compared with those before treatment, oxygen index (OI) in group A decreased progressively (P <0.05); OI in group B showed an upward trend Points also showed an upward trend, and there was a significant difference at each time point. In group C, OI increased first (1,2 h significantly higher than the other groups) and then decreased sharply (significantly lower than the other groups at 3,4 h). E Group 3,4h significantly higher than the other groups. In group A, the mPAP showed an upward trend at all time points, which was significantly higher than that before treatment; while in groups B, D and E, the levels of mPAP tended to decrease, Former and other groups. The scores of lung injury in group C were higher than those in other groups (P (0.05), the lowest in group E, and the score of lung injury in groups B and D was significantly lower than that in group A. Conclusion: Protective pulmonary ventilation combined with anisodamine Animal models of ALI have synergistic therapeutic effects.