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白蛋白是肝脏表达的血清蛋白 ,其组织特异性表达的基础在于白蛋白基因存在特异性调控序列如启动子、增强子 ,可与肝脏富含的转录因子如 HNF1、C/ EBP及 e H- TF等特异性结合 ,刺激基因高效转录。自 1 989年 Izban克隆并提交了小鼠白蛋白调控序列 ,其在转基因动物及基因治疗中得到了广泛的应用 ,实现了基因转移的器官靶向性。近年的研究发现 ,与其他启动子如 CMV、PGK相比 ,白蛋白启动子引导的基因转移引起的免疫反应较轻 ,并可克服因启动子失活而导致的转基因沉默现象 ,相信随着转基因技术的不断发展 ,其将有越来越广泛的应用
Albumin is a serum protein expressed in the liver. Its tissue-specific expression is based on the existence of specific regulatory sequences of albumin genes such as promoters and enhancers, which can interact with liver-rich transcription factors such as HNF1, C / EBP and eH- TF and other specific binding to stimulate efficient gene transcription. Since 1978, Izban cloned and submitted mouse albumin regulatory sequences, which have been widely used in transgenic animals and gene therapy, and have achieved organ-targeted gene transfer. Recent studies have found that albumin promoter-induced gene transfer can cause less immune response than other promoters such as CMV and PGK and can overcome the phenomenon of gene silencing caused by inactivation of the promoter. It is believed that as the transgene The continuous development of technology, it will have more and more widespread application