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目的以树突状细胞（DC）在体外诱导抗肝癌免疫。方法自肝癌患者外周血中分离DC；以粒／巨噬细胞集落刺激因子（GM－CSF）及白介素-4（IL－4）联合刺激DC；以人肝癌细胞系HepG2细胞的肿瘤相关抗原（TAA）激活DC；DC诱导自体T淋巴细胞增殖、分化为细胞毒性T细胞（CTL）；检测CTL及其上清液对HepG2细胞、BEL－7402细胞、LOVO细胞及HOS－8603细胞的细胞毒作用。结果经人肝癌细胞系HePG2细胞的TAA激活并经GM－CSF及IL－4联合刺激后，肝癌患者外周血DC能够诱导自体T淋巴细胞增殖分化为CTL，该CTL及其上清液对HePG2细胞均有高效杀伤作用（杀伤率分别为92％±10％和41％±8％），对BEL－7402细胞亦有较强的杀伤力（杀伤率分别为56％±10％和31％±9％），对SGC－7901细胞、LOVO细胞及HOS－8603细胞则无明显的细胞毒作用。结论肝癌患者外周血DC体外能够诱导高效而特异抗肝癌免疫。提示DC可能在肿瘤治疗及预防其复发和转移中发挥重要作用。
Objective To induce anti-hepatocellular carcinoma immunity in vitro by dendritic cells (DC). METHODS: DCs were isolated from peripheral blood of patients with hepatocellular carcinoma; DCs were stimulated with GM-CSF and interleukin-4 (IL-4); tumor-associated antigens (TAA) of human hepatoma cell line HepG2 cells were used. DCs were activated; DCs induced proliferation of autologous T lymphocytes and differentiated into cytotoxic T cells (CTL); cytotoxic effects of CTLs and their supernatants on HepG2 cells, BEL-7402 cells, LOVO cells, and HOS-8603 cells were examined. Results After activated by TAA of human hepatocellular carcinoma cell line HePG2 cells and stimulated by GM-CSF and IL-4, peripheral blood DCs of hepatocellular carcinoma cells could induce the proliferation and differentiation of autologous T lymphocytes into CTLs. The CTLs and their supernatants correspond to HePG2 cells. All of them have high killing effect (92%±10% and 41%±8%, respectively), and also have a strong killing effect on BEL-7402 cells (kill rate is 56%±10% and 31%±9, respectively). %)) No significant cytotoxicity was observed on SGC-7901 cells, LOVO cells, and HOS-8603 cells. Conclusions Peripheral blood DCs of liver cancer patients can induce efficient and specific anti-hepatocellular carcinoma immunity in vitro. It suggests that DC may play an important role in the treatment of tumors and their prevention of recurrence and metastasis.