大葱(Allium fistulosum)广泛栽培,花期长,可不断取到花序,是观察减数分裂的好材料。大葱开花时,只要花序外有嫩绿总苞包裹,均可取下花序,用卡诺固定液(冰醋酸:无水酒精=1∶3)固定。2—12小时后若不马上观察,可将材料移入70％酒精里保存备用;若放置在4℃低温冰箱中保存更好。观察时取下花序,分别在50％、30％酒精液中过渡,直至用蒸馏水冲洗无酒精气味。从花序上取下花药放在载玻片上,以快刀片从花药中央横切,镊子挤压,去掉药壁残渣,滴上改良品红液1—2滴染色(醋酸洋红、地衣红、苏木精均可),5—7分钟后盖上盖片,用橡皮头轻压盖片,使花粉母细胞均匀散开,即行观察。若染 Allium fistulosum is widely cultivated, with long flowering and inflorescence, which is a good material for observing meiosis. When green onions, as long as inflorescence tender green capsule wrapped, can be removed inflorescence, fixed with Carnot's solution (glacial acetic acid: anhydrous alcohol = 1: 3). If not observed immediately after 2-12 hours, the material can be stored in 70% alcohol reserve; if placed in 4 ℃ low temperature refrigerator to save better. The inflorescences were removed during the observation and transitioned respectively in 50%, 30% alcohol solution until no alcoholic odor was rinsed with distilled water. Remove the anthers from the inflorescence on the slide, with a quick blade cross from the anther central, squeeze the forceps, remove the drug wall residue, drops of modified magenta liquid 1-2 drops of staining (acetic acid magenta, lichen red, hematoxylin Fine can be), 5-7 minutes after the cover, cover with a rubber head light cover, the pollen mother cells evenly spread, the line observation. If dyed