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目的探讨EB病毒mir-BART5可促进鼠成纤维细胞恶性表征的作用机制。方法生物信息学分析显示miR-BART5与miR-18序列相近,miR-18的靶基因表达的结缔组织生长因子(CTGF)可促进细胞增殖。在鼠成纤维细胞NIH/3T3中瞬时转染mir-BART5-mimics使其表达上调,采用Western blot检测对照组与转染组细胞的CTGF表达,采用体外软琼脂集落生成试验观察两组细胞生长的恶性表征。结果转染组细胞在软琼脂上呈恶性表征。CTGF在转染组中表达上调(P<0.05),导致NIH/3T3细胞增殖能力增强。结论 EBV病毒miR-BART5通过调控miR-18途径上调CTGF的表达,从而促进细胞的恶性表征。
Objective To investigate the mechanism of EBV mir-BART5 in promoting the malignant characterization of murine fibroblasts. Methods Bioinformatics analysis showed that miR-BART5 is similar to miR-18 sequence, and CTGF expressed by miR-18 target gene can promote cell proliferation. The expression of mir-BART5-mimics was transiently transfected into mouse fibroblasts NIH / 3T3. The expression of CTGF in control group and transfected group was detected by Western blot. The in vitro soft agar colony formation assay was used to observe the cell growth Malignant characterization. Results The transfected cells showed malignant features on soft agar. The expression of CTGF was up-regulated in transfection group (P <0.05), leading to the enhancement of NIH / 3T3 cell proliferation. Conclusion EBV virus miR-BART5 promotes the malignant characterization of the cells by up-regulating the expression of CTGF by regulating the miR-18 pathway.