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目的：为了满足地苷洛韦（脱氧无环鸟苷，ＤＣＶ）药代动力学研究和血药浓度监测的需要，建立了能够同时测定血中ＤＣＶ和阿苷洛韦（无环鸟苷，ＡＣＶ）的反相高效液相色谱（ＨＰＬＣ）分离测定方法。方法：血浆经离心式超滤器离心除蛋白后，取超滤液直接进行ＨＰＬＣ分析。色谱条件如下：ＳｐｈｅｒｉｓｏｒｂＣ１８分析柱；甲醇水（１５８５，体积比）作为流动相，流速０．７ｍｌ／ｍｉｎ；可编程紫外检测器，波长：ＤＣＶ３０５ｎｍ，ＡＣＶ２０５ｎｍ；外标法定量。结果：血中ＤＣＶ和ＡＣＶ的最低检出浓度分别是０．１４和０．０８μｇ／ｍｌ；在１．０～２０μｇ／ｍｌ浓度范围内，血中ＤＣＶ和ＡＣＶ测定的平均回收率分别是（９９．２±１０．４）％和（１０９．２±２．５）％，重复测定变异系数分别是２．７％～１０．７％和０．２％～４．０％。结论：建立了同时测定血中ＤＣＶ和ＡＣＶ浓度的ＨＰＬＣ分离测定方法。该法灵敏、快速、准确、复演性好。
OBJECTIVE: To meet the need of pharmacokinetics study and plasma concentration monitoring of dicallozide (DCA), a method was developed for the simultaneous determination of DCV and alopecuvir (acyclovir, ACV ) Reversed-phase high performance liquid chromatography (HPLC) separation assay. Methods: The plasma was centrifuged by centrifugal ultrafilter to remove protein, and the ultrafiltrate was directly analyzed by HPLC. Chromatographic conditions are as follows: SpherisorbC18 analytical column; methanol water (1585, volume ratio) as a mobile phase, flow rate 0.7ml / min; programmable UV detector, wavelength: DCV305nm, ACV205nm; Results: The lowest detectable concentrations of DCV and ACV in blood were 0.14 and 0.08μg / ml, respectively. The average recoveries of DCV and ACV in blood were (99%) in the range of 1.0 ~ 20μg / ml .2 ± 10.4)% and (109.2 ± 2.5)% respectively. The coefficient of variation of repeated measurement was 2.7% -10.7% and 0.2% -4.0% respectively. Conclusion: A HPLC method for simultaneous determination of DCV and ACV in blood was established. The method is sensitive, fast, accurate, good rehearsal.