复方亚甲蓝注射液系亚甲蓝与盐酸普鲁卡因的混合溶液,本文采用一阶导数吸收度法取229.50及214.00nm波长处的峰、谷吸收度绝对值之和作为制剂中盐酸普鲁卡因的定量信息,求得相关系数γ=0.9993,平均回收率为99.4%,变异系数CV%=±0.83%。 Compound methylene blue injection solution is a mixture of methylene blue and procaine hydrochloride. In this paper, the first-order derivative absorption method was used to obtain the sum of the absolute values ​​of the peak and valley absorption at wavelengths of 229.50 and 214.00 nm. The quantitative information of rocaine obtained the correlation coefficient γ = 0.9993, the average recovery was 99.4%, the coefficient of variation CV% = ± 0.83%.