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以人肝庙细胞株ＧＨＣ－３细胞为抗原，免疫制备获得抗人肝癌细胞膜单克隆抗体ＨＬＭ＿３（ＨＬＭ＿３－ＭｃＡｂ）．应用ＨＬＭ＿３－ＭｃＡｂ研究分析肝瘤细胞表面抗原性质及其对ＧＨＣ－３细胞的生物效应，结果表明：ＨＬＭ＿３－ＭｃＡｂ可抑制已铺展的ＧＨＣ－３细胞生长；辣根过氧化物酶结合法探测受ＭｃＡｂ作用的细胞ＣｏｎＡ受体和ｒａｓ瘤基因产物ｐ２１．反应均明显减弱；交叉－阻断测试抗原及ＣｏｎＡ受体发现，无论是细胞先与ＭｃＡｂ结合后再与ＣｏｎＡ反应，抑或先与ＣｏｎＡ反应后再与ＭｃＡｂ结合，都显示ＧＨＣ－３细胞的膜抗原与腹表面的ＣｏｎＡ受体有密切关系。进一步作糖化学分析，从高效薄层色谱（ＨＰＴＬＣ）区带和扫描初步显示为不完全分化的中性糖脂。
The anti-human hepatoma cell membrane monoclonal antibody HLM_3 (HLM_3-McAb) was prepared by immunizing with human liver cell line GHC-3 cells as antigen. HLM_3-McAb was used to study the characteristics of hepatoma cell surface antigens and its biological effects on GHC-3 cells. The results showed that: HLM_3-McAb can inhibit the growth of spread GHC-3 cells; detection of horseradish peroxidase binding method McAb-activated cell ConA receptor and ras tumor gene product p21. The response was significantly attenuated; cross-blocking of test antigens and ConA receptors revealed that both cells first bind to McAb and then react with ConA, or they react with ConA and then bind to McAb. Both show membrane antigens of GHC-3 cells. It is closely related to the ConA receptor on the ventral surface. Further glycochemical analysis was initially performed as shown by HPTLC zones and scans as incompletely differentiated neutral glycolipids.