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本文采用玻璃微电极细胞外记录法,观察正常大鼠和6-羟多巴胺(6-hydroxydopamine,6-OHDA)损毁黑质致密部大鼠杏仁基底外侧核(basolateral nucleus,BL)神经元电活动的变化,以及体循环给予选择性5-HT1A受体拮抗剂WAY-100635对神经元电活动的影响。结果显示,正常大鼠BL投射神经元和中间神经元的放电频率分别是(0.39±0.04)Hz和(0.83±0.16)Hz,6-OHDA损毁大鼠BL投射神经元和中间神经元的放电频率分别是(0.32±0.04)Hz和(0.53±0.12)Hz,与正常大鼠相比无显著差异。在正常大鼠,所有投射神经元呈现爆发式放电;94%的中间神经元为爆发式放电,6%为不规则放电。在6-OHDA损毁大鼠,85%的投射神经元呈现爆发式放电,15%为不规则放电;86%的中间神经元为爆发式放电,14%为不规则放电,与正常大鼠相比无显著差别。静脉给予0.1mg/kg体重的WAY-100635不改变正常大鼠和6-OHDA损毁大鼠BL投射神经元和中间神经元的放电频率。然而,0.5mg/kg体重的WAY-100635却显著降低正常大鼠BL投射神经元的平均放电频率(P<0.01),明显增加6-OHDA损毁大鼠BL投射神经元的平均放电频率(P<0.004)。高剂量WAY-100635不影响正常大鼠和6-OHDA损毁大鼠BL中间神经元的平均放电频率。结果表明,黑质多巴胺能损毁后内在和外在的传入调节BL神经元的活动,在正常大鼠和6-OHDA损毁大鼠5-HT1A受体调节投射神经元的活动,并且在6-OHDA损毁大鼠WAY-100635诱发投射神经元平均放电频率增加。结果提示,5-HT1A受体在帕金森病情感性症状的产生中起重要作用。
In this study, we used glass microelectrode extracellular recording method to observe the electrical activity of basolateral nucleus (BL) neurons in normal rat and 6-hydroxydopamine (6-OHDA) -based substantia nigra pars compacta rats Changes, and systemic effects of selective 5-HT1A receptor antagonist WAY-100635 on the electrical activity of neurons. The results showed that the discharge frequency of BL projection neurons and interneurons in normal rats were (0.39 ± 0.04) Hz and (0.83 ± 0.16) Hz, respectively. The discharge frequency of BL projection neurons and interneurons in 6-OHDA lesioned rats (0.32 ± 0.04) Hz and (0.53 ± 0.12) Hz respectively, no significant difference compared with normal rats. In normal rats, all projecting neurons showed explosive discharges; 94% of the interneurons were explosive and 6% were irregularly discharged. In 6-OHDA-lesioned rats, 85% of projecting neurons showed explosive discharge, 15% were irregular discharge; 86% of interneurons were explosive discharge, 14% of irregular discharge, compared with normal rats No significant difference. WAY-100635 intravenously administered at a dose of 0.1 mg / kg did not change the firing rate of BL projection neurons and interneurons in normal and 6-OHDA-lesioned rats. However, WAY-100635 at 0.5 mg / kg body weight significantly reduced the mean firing frequency of BL projection neurons in normal rats (P <0.01), and significantly increased the average firing frequency of BL projection neurons in 6-OHDA damaged rats (P < 0.004). High dose WAY-100635 did not affect the average discharge frequency of BL interneurons in normal and 6-OHDA-lesioned rats. The results show that dopaminergic nigra dopamine impairs intrinsic and extrinsic afferent modulation of BL neuronal activity and regulates 5-HT1A receptor-mediated neuronal activity in normal and 6-OHDA-lesioned rats, and in 6- OHDA lesioned rats WAY-100635-induced increase in the average firing frequency of projecting neurons. The results suggest that 5-HT1A receptor plays an important role in the development of sensitivities in Parkinson’s disease.