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对人体血清蛋白质(HSA)的吸附进行了热效应和动力学的研究,按照其在准均匀介质中的扩散过程,描述了HSA吸附动力学,研究表明该过程服从方程a=kL1/2,在研究合成活性炭(SCN)对HSA的吸附时(在磷酸盐缓冲液中),发现该过程伴随有放热行为(每吸附一克蛋白质放热约为50焦耳)。由放热速率和蛋白质吸附速率之比即((?)_q/(?)_τ)/((?)_a/(?)_τ)来表达热效应测量结果。研究了温度、离子强度、起始蛋白质浓度对((?)_q/(?)_a)_τ时间变量的影响,研究表明在被吸附的HSA分子中没有明显的结构形态变化的部分随着起始溶质浓度和离子强度的增大而增加。
The adsorption kinetics of human serum protein (HSA) and its kinetics were investigated. The adsorption kinetics of HSA was described according to its diffusion process in a quasi-homogeneous medium. The results showed that this process obeys the equation a = kL1 / 2, Synthetic Activated Carbon (SCN) adsorbed HSA (in phosphate buffer) and found to be accompanied by exothermic behavior (about 50 joules per 1 g protein adsorption). Thermal effect measurements were expressed as the ratio of the exothermic rate to the rate of protein adsorption, ((?) _ Q / (?) _ Τ) / ((?) _ A / (?) _?). The effects of temperature, ionic strength and initial protein concentration on the time variable of (?) _ Q / (?) _a) _τ were studied. The results showed that there was no obvious change in the structure of the adsorbed HSA molecule with the onset Solute concentration and ionic strength increases.