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以施用化肥225 kg/hm2为对照,设置两种不同的沼液用量(以纯氮计):沼液I(168 kg/hm2)和沼液II(225 kg/hm2),通过连续3 a的甘蓝种植试验,研究沼液对甘蓝连作土壤微生物区系、土壤酶活性以及土壤养分与土壤酶活性之间的相关性.结果表明:1)施用沼液显著改善了连作土壤的微生物区系,溶磷细菌、解钾细菌、氨化细菌、固氮菌和放线菌的数量显著增加;同时抑制了真菌的富集.两种不同用量的沼液处理较化肥处理的土壤细菌/真菌(B/F)值分别提高了142.7%和202.3%.2)在225 kg/hm2等氮水平下,施用沼液显著提高了土壤的蔗糖酶、磷酸酶和蛋白酶活性,较之化肥处理分别提高了63.96%、137.61%和139.66%;同时显著降低了土壤过氧化氢酶和多酚氧化酶活性.3)土壤养分与土壤酶活性相关性分析表明,有机质含量与土壤脲酶、磷酸酶、蔗糖酶和蛋白酶活性呈显著正相关,与过氧化氢酶活性呈显著负相关,而与多酚氧化酶活性无明显相关性;磷酸酶活性与土壤速效磷含量呈显著正相关;蛋白酶活性与土壤碱解氮、全氮含量呈显著正相关.表4参24
Using 225 kg / hm2 of chemical fertilizers as control, two different biogas volumes (pure nitrogen) were set: biogas slurry I (168 kg / hm2) and biogas slurry II (225 kg / hm2) The experiment was carried out to study the correlation between biogas slurry and soil microbial activities, soil enzyme activities and soil nutrients and soil enzyme activities in continuous cropping of cabbage.The results showed that: 1) biogas slurry application significantly improved the microbial flora, Bacteria, potassium-releasing bacteria, ammonifying bacteria, azotobacter and actinomycetes significantly increased, while the enrichment of fungi was inhibited. Two kinds of biogas slurry with different dosages were used to treat soil bacteria / fungi (B / F) (142.7% and 202.3% respectively) .2) Under 225 kg / hm2 and other nitrogen levels, the application of biogas slurry significantly increased the activity of sucrase, phosphatase and protease in soil, which increased by 63.96%, 137.61 % And 139.66%, respectively. At the same time, the activities of soil catalase and polyphenol oxidase were significantly decreased.3) The correlation analysis between soil nutrients and soil enzyme activities showed that the contents of organic matter and soil urease, phosphatase, sucrase and protease activities were significantly Positive correlation with catalase activity was significantly negative But no significant correlation with the activity of polyphenol oxidase.The activity of phosphatase was positively correlated with the content of available phosphorus in soil and the activity of protease was positively correlated with the content of alkaline hydrolyzable nitrogen and total nitrogen in soil