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分析研究不同部位及产地穿心莲药材,为穿心莲相关制剂工艺提供依据。以穿心莲内酯峰为参照,确定了27个共有指纹峰,建立了23批穿心莲药材的UPLC-PDA数字化定量指纹图谱,特征提取后对其进行主成分分析(PCA);采用外标法测定23批穿心莲药材中穿心莲内酯、新穿心莲内酯、去氧穿心莲内酯、脱水穿心莲内酯成分含量,并采用Plackett-Burman试验设计结合pareto图研究耐用性因素对方法的影响。结果发现,穿心莲药材部位比产地对药材质量的影响更显著;药材不同部位4种内酯含量差异大;流动相p H是影响方法耐用性较大的因素。
Analysis of different parts and origin Andrographis medicine, preparation of Andrographis preparation technology to provide the basis. Based on the andrographolide peaks, 27 common fingerprint peaks were identified, and UPLC-PDA quantitative fingerprint was established for 23 batches of Andrographis paniculata. PCA was extracted after characteristic feature extraction. The external standard method was used to determine 23 The contents of andrographolide, neoandrographolide, andrographolide and dehydroandrographolide in Andrographis paniculata were determined. The Plackett-Burman experimental design and pareto plot were used to study the effect of durability factors on the method. The results showed that the parts of Andrographis than the place of origin on the quality of the medicinal material is more significant; different parts of the four lactones content difference; mobile phase p H is the method of influencing the greater durability.