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建立水产品中氯霉素的含量测定方法。采用气相色谱程序升温法测定,以安捷伦DB-1701毛细管色谱柱(30 m×0.32 mm×0.25μm),汽化室温度260℃,检测器温度300℃,程序升温(起始温度100℃,保持2 min,以10℃/min升至250℃,保持5 min)。结果表明,氯霉素在该色谱条件下氯霉素分离良好,其线性范围为0.002 5~0.050 0μg·mL-1,相关系数均为0.999 5,检出限分别为0.1μg·kg-1;样品加样回收率为91.3%~97.3%,RSD为2.2%。该方法灵敏、准确、可靠,具有分析时间短、抗干扰性好、定量准确等优点,可为水产品中氯霉素残留测定方法开发提供科学数据。
Establishment of aquatic products in chloramphenicol determination. The temperature of the column was set at 30 ℃, the temperature of the chamber was set at 300 ℃, the temperature was programmed at 100 ℃, and kept at 2 min, raised to 250 ° C at 10 ° C / min for 5 min). The results showed that chloramphenicol was separated well under the chromatographic conditions. The linear range was 0.002 5 ~ 0.050 0 μg · mL-1, the correlation coefficient was 0.999 5, the detection limits were 0.1 μg · kg-1, respectively. The sample recovery was 91.3% ~ 97.3% with RSD of 2.2%. The method is sensitive, accurate and reliable, and has the advantages of short analysis time, good anti-interference, and accurate quantification. It can provide scientific data for the development of determination method of chloramphenicol residues in aquatic products.