论文部分内容阅读
目的研究胰升血糖素样肽-1(GLP-1)对波动性高糖诱导大鼠胰岛细胞增殖功能的影响及机制。方法将获取的原代胰岛细胞分为5组,即正常葡萄糖(5.5mmol/L)组、恒定高糖(30.0mmol/L)组、波动高糖(每24h轮换培养于5.5、30.0mmol/L)组、恒定高糖+GLP-1(100nmol/L)组和波动高糖+GLP-1组。干预7d后检测细胞增殖活性、活性氧簇(ROS)、细胞周期及周期蛋白cyclinD1、p21、p27、Skp2的表达。结果 GLP-1干预可降低波动性高糖诱导的细胞内ROS水平[(194.40±19.20)vs(406.78±18.40),P<0.05],上调促细胞周期cyclinD1、Skp2表达,下调周期抑制蛋白p21、p27表达,使停滞在G0/G1期的细胞比例减少,改善细胞增殖活性[(1.38±0.09)vs(0.44±0.10),P<0.05]。结论GLP-1可通过降低氧化应激水平及对不同细胞周期蛋白表达的调节改善波动性高糖抑制的胰岛细胞增殖活性。
Objective To investigate the effect and mechanism of GLP-1 on proliferation of pancreatic islet cells induced by high glucose in rats. Methods Primary islet cells were divided into 5 groups: normal glucose (5.5mmol / L) group, constant high glucose (30.0mmol / L) group, fluctuating high glucose ) Group, constant high glucose + GLP-1 (100nmol / L) group and high glucose + GLP-1 group. After 7 days of intervention, the cell proliferative activity, the reactive oxygen species (ROS), the cell cycle and the expressions of cyclinD1, p21, p27 and Skp2 were detected. Results The GLP-1 intervention could reduce the level of intracellular ROS induced by high glucose ([(194.40 ± 19.20) vs (406.78 ± 18.40), P <0.05], and upregulate the expression of cyclinD1 and Skp2 and downregulate the expression of p21, p27 expression decreased the proportion of cells arrested in G0 / G1 phase and improved cell proliferation activity (1.38 ± 0.09 vs 0.44 ± 0.10, P <0.05). Conclusion GLP-1 can improve the proliferation activity of pancreatic islet cells with high glucose and high glucose by decreasing the level of oxidative stress and regulating the expression of different cell cycle proteins.