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目的:探讨银杏叶提取物对实验性大鼠肝纤维化的预防及治疗作用机制. 方法:采用四氯化碳腹腔注射诱导的大鼠肝纤维化模型, 60只Wistar大鼠分为5组:模型组、银杏叶干预组、银杏叶治疗组、银杏叶组,另设正常对照组,应用HE染色观察大鼠肝组织的改变及Von-Gieson胶原纤维特殊染色观察肝纤维化程度,并采用免疫组织化学染色法及RT-PCR法检测肝组织Ⅰ型胶原、TGF-β1蛋白和mRNA的表达. 结果:银杏叶干预及治疗组与模型组相比肝组织结构明显改善、纤维化增生程度减轻;肝组织内Ⅰ型胶原、TGF-β1 的含量(Ⅰ型胶原:0.2563±0.0009 vs 0.2885±0.0025, 0.2541±0.0076 vs 0.2885±0.0025,P<0.01;TGF- β1:0.2785±0.0012 vs 0.3015±0.0012.0.2791±0.0016 vs 0.3015±0.0012,P<0.01)以及mRNA的表达均明显低于模型组(Ⅰ型胶原:0.0778±0.054 vs 0.2361±0.113,0.1075±0.007 vs 0.2361±0.113,P<0.01; 0.523±0.015 vs 0.956±0.049,0.524±0.009 vs 0.956±0.049,P<0.01);银杏叶组与正常对照组之间无差异. 结论:银杏叶提取物可抑制肝星状细胞激活和转化,下调Ⅰ型胶原、TGF-β1蛋白质及其mRNA的表达,从而抑制或逆转纤维化形成.
Objective: To investigate the preventive and therapeutic mechanism of Ginkgo biloba extract on experimental liver fibrosis in rats. Methods: Rat models of liver fibrosis induced by intraperitoneal injection of carbon tetrachloride were used. Sixty Wistar rats were divided into five groups: Model group, Ginkgo biloba intervention group, Ginkgo biloba leaf treatment group, Ginkgo biloba leaf group, and normal control group were used. HE staining was used to observe the changes of rat liver tissue and the special staining of Von-Gieson collagen fibers was used to observe the degree of hepatic fibrosis. Histochemical staining and RT-PCR were used to detect the expression of type I collagen, TGF-β1 protein and mRNA in liver tissue. Results: Compared with the model group, the structure of liver tissue was significantly improved and the degree of fibroplasia was alleviated in Ginkgo biloba leaves intervention group and treatment group. Content of type I collagen and TGF-β1 in liver tissue (type I collagen: 0.2563±0.0009 vs. 0.2885±0.0025, 0.2541±0.0076 vs. 0.2885±0.0025, P<0.01; TGF-β1: 0.2785±0.0012 vs. 0.3015±0.0012.0.279 (±0.0016 vs 0.3015±0.0012, P<0.01) and mRNA expression were significantly lower than those in the model group (type I collagen: 0.0778±0.054 vs 0.2361±0.113, 0.1075±0.007 vs 0.2361±0.113, P<0.01; 0.523±0.015 vs 0.956±0.049, 0.524±0.009 vs 0.956± 0.049, P <0.01); Ginkgo biloba leaves group and normal control group no difference. Conclusion: Ginkgo biloba extract can inhibit the activation and transformation of hepatic stellate cells, down-regulate the expression of type I collagen, TGF-β1 protein and its mRNA, In order to inhibit or reverse the formation of fibrosis.