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Objective:To investigate the expression of Tbx2 protein in pancreatic cancer tissues and its molecular regulation mechanism by Wnt/β-catenin signaling.Methods:49 pancreatic cancer and 13 non-cancer tissue specimens were obtained and examined the expression of Tbx2,and the correlation between the expression of Tbx2 and clinicopathological parameters was analyzed.The immunohistochemistry,immunocytochemistry,RT-PCR and Western blot assay methods were used to detect the changes of expression levels of β-catenin and Tbx2.Results:Tbx2 was amplified in 34 of 49 pancreatic cancers,and in 13 non-cancer tissues,only one sample amplified.The further study demonstrated that Tbx2 had a significant positive correlation with tumor differentiation degree and clinical stage,but it did not relate to the sex,age and the disease region.Inhibition of β-catenin degradation through the treatment of pancreatic cancer cells SW1990 with different concentrations of lithium chloride indicated that accumulation of β-catenin was sufficient to induce TBX2 expression.Conclusion:TBX2 gene plays an important role in the occurrence and development of pancreatic cancer and the accumulation of β-catenin contributes to the expression of TBX2.The Wnt/β-catenin signaling pathway participates the regulation of TBX2 in pancreatic cancer cells.