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目的探讨小儿肾母细胞瘤组织中E2F3的表达与肾母细胞瘤临床分期的相关性。方法利用免疫组织化学方法检测26例肾母细胞瘤组织中E2F3阳性细胞的百分比、积分光密度及E2F3 mRNA的表达,比较采用t检验及方差分析,P<0.05有统计学意义。结果 E2F3在所有肾母细胞瘤组织中均过度表达,在肾母细胞瘤侵蚀性强时,E2F3蛋白及mRNA的表达水平较高。结论 E2F3在小儿肾母细胞瘤组织中过度表达,E2F3表达水平与肾母细胞瘤临床分期密切相关。
Objective To investigate the correlation between the expression of E2F3 and the clinical stage of Wilms’ tumor in pediatric nephroblastoma. Methods Immunohistochemistry was used to detect the percentage of E2F3 positive cells, the integral optical density and the expression of E2F3 mRNA in 26 cases of nephroblastoma. Compared with t test and analysis of variance, P <0.05 was statistically significant. Results E2F3 was overexpressed in all nephroblastoma tissues, and E2F3 protein and mRNA were highly expressed in nephroblastoma with strong aggressiveness. Conclusion E2F3 is overexpressed in pediatric nephroblastoma tissue. The expression of E2F3 is closely related to the clinical stage of Wilms’ tumor.