论文部分内容阅读
目的 分析广州地区SARS流行期和非流行期无偿献血人群中严重急性呼吸道综合征(SARS)病毒感染 情况,为制定预防输血传播SARS病毒措施提供科学依据。方法 采用酶联免疫吸附试验(ELISA)对无偿献血者血 液进行SARS病毒抗体筛查,对SARS病毒抗体阳性样本用荧光聚合酶链反应(PCR)法进一步检测SARS病毒核 酸。采用统一的个案调查表对20名SARS病毒抗体阳性无偿献血者进行电话咨询调查,同时对31名SARS康复献 浆者进行检测,分析相关数据作对照。结果 6120名无偿献血者中,共检测出SARS病毒抗体阳性56例,阳性率为 0.92%,31名SARS康复献浆者中,检测出SARS病毒抗体阳性30例,阳性率为96.77%;SARS流行期和非流行期 无偿献血者SARS病毒抗体阳性率分别为0.91%和0.92%,56名无偿献血者SARS病毒抗体阳性的平均S/CO值 (2.34)和抗体平均滴度(≤1∶2)均明显低于30名SARS康复献浆者的平均S/CO值(14.8)和抗体平均滴度(≤1∶ 32);56例SARS病毒抗体阳性无偿献血者血液样本均未检测出SARS病毒核酸;20例SARS病毒抗体阳性无偿献 血者的调查显示:献血者身体健康,无SARS患者密切接触史。结论 广州地区无偿献血人群中存在的低水平 SARS病毒抗体阳性率,是否表明SARS病毒抗体阳性献血者曾经感染过SARS病毒,尚需进一步
Objective To analyze the prevalence of severe acute respiratory syndrome (SARS) infection among the blood donors in the non-epidemic and non-epidemic phases of SARS in Guangzhou, and to provide a scientific basis for the development of measures to prevent the spread of SARS virus. Methods Serum samples of SARS virus were screened by enzyme-linked immunosorbent assay (ELISA). The samples of SARS virus were further tested by fluorescence polymerase chain reaction (PCR) to detect SARS virus nucleic acid. A total of 20 SARS-HIV-positive donors were surveyed by telephone using a unified case-control questionnaire. At the same time, 31 SARS rehabilitation donors were tested and the related data were analyzed. Results Of 6120 unpaid blood donors, 56 cases were positive for SARS virus, the positive rate was 0.92%. Out of 31 SARS donors, 30 cases were positive for SARS virus, the positive rate was 96.77%. SARS prevalence The positive rates of antibody against SARS in unpaid blood donors were 0.91% and 0.92%, respectively. The mean S / CO value (2.34) and mean antibody titer (≤1: 2) Were significantly lower than the average S / CO value (14.8) and the average antibody titer (≤1: 32) in 30 SARS rehabilitation donors; no SARS virus nucleic acid was detected in the blood samples from 56 SARS-positive blood donors ; 20 cases of SARS virus-positive blood donors survey showed that: blood donors in good health, no SARS patients close contact history. Conclusions The positive rate of low level SARS virus in unpaid blood donors in Guangzhou indicates whether SARS virus-positive donors have ever been infected with SARS virus,