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采用简单重复序列(SSR)的分析方法,对来自安徽和黑龙江省的大豆疫霉群体进行了遗传多样性分析。通过使用20对SSR引物对供试的83株大豆疫霉菌株进行PCR扩增,共得到109个SSR标记,全部为多态性标记,平均每对引物扩增出5.5条带。遗传变异与相似性分析表明,安徽群体具有更高的遗传变异度,安徽群体与黑龙江群体间遗传相似性较低;聚类分析显示,供试菌株在80%的相似性水平上可被区分为7个类群,且安徽群体分布于更多的聚类组中;Shannon-Wiener多样性指数也表明安徽群体的遗传多样性较黑龙江群体丰富。综合分析表明,本研究的结果不支持关于安徽的大豆疫霉可能来源于黑龙江的推测。
Genetic diversity of P. sojae populations from Anhui and Heilongjiang Provinces was analyzed by Simple Repeat Sequencing (SSR). A total of 109 SSR markers were obtained by PCR amplification of 83 strains of Phytophthora sojae using 20 pairs of SSR primers. All of them were polymorphic markers, with an average of 5.5 bands per primer pair. Genetic variation and similarity analysis showed that the Anhui population had higher genetic variation, and the genetic similarity between Anhui population and Heilongjiang population was lower. Cluster analysis showed that the tested strains could be divided into 80% similarity 7 groups, and Anhui groups were distributed in more cluster groups. The Shannon-Wiener diversity index also showed that the genetic diversity of Anhui population was more abundant than that of Heilongjiang group. Comprehensive analysis shows that the results of this study do not support the speculation that Phytophthora sojae in Anhui may originated from Heilongjiang.