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目的检测microRNA-199a-3p(miR-199a-3p)在肾癌细胞株和组织中的表达情况并探究miR-199a-3p在肾癌细胞中的作用。方法利用实时定量RT-PCR检测miR-199a-3p在肾癌细胞和组织中的表达水平;利用miR-199a-3p模拟物转染肾癌细胞786-0上调miR-199a-3p后,通过CCK-8、克隆形成、Transwell以及细胞周期检测来探究其在肾癌细胞中的作用。结果 miR-199a-3p在肾癌细胞中明显低表达,在78%(14/18)的肾癌组织中亦明显低表达;上调miR-199a-3p可显著抑制肾癌细胞的增殖、存活和侵袭并能诱导细胞周期G1期阻滞。结论我们的研究显示在肾癌中miR-199a-3p明显低表达并参与肾癌的发生、发展,这表明miR-199a-3p具有作为肾癌诊断和治疗靶点的潜能。
Objective To detect the expression of microRNA-199a-3p (miR-199a-3p) in renal cell carcinoma cell lines and tissues and to explore the role of miR-199a-3p in renal cell carcinoma. The expression of miR-199a-3p was detected by real-time quantitative RT-PCR in renal cell carcinoma and tissues. MiR-199a-3p mimics were transfected into 786-0 cells to up-regulate the expression of miR-199a-3p. -8, clone formation, Transwell, and cell cycle assays to explore its role in renal cancer cells. Results miR-199a-3p was significantly downregulated in renal carcinoma cells and significantly lower in 78% (14/18) of renal carcinoma tissues. Upregulation of miR-199a-3p significantly inhibited the proliferation and survival of renal carcinoma cells Invasion and can induce cell cycle G1 arrest. Conclusions Our study shows that miR-199a-3p is significantly down-regulated in renal cell carcinoma and is involved in the development and progression of renal cell carcinoma. This indicates that miR-199a-3p has potential as a target for diagnosis and treatment of renal cell carcinoma.