近年来,免疫组化技术已成为病理研究和临床病理诊断的重要方法,但某些抗体特别是癌基因抗体用常规免疫组化染色效果不理想。我们利用微波(MW)产生的超高速震动及穿透效应,以加速抗原抗体结合反应为原理,建立了一种快速MW-SP法免疫组化染色技术。经一年多近千张切片微波免疫组化染色的应用,效果满意,现介绍如下。 In recent years, immunohistochemistry has become an important method for pathological studies and clinicopathological diagnosis. However, some antibodies, especially oncogene antibodies, are less effective than routine immunohistochemical staining. Using the principle of ultra-high-speed vibration and penetration through microwave to accelerate the antigen-antibody binding reaction, we established a rapid MW-SP immunohistochemical staining technique. After more than a year of nearly a thousand slices of microwave immunohistochemical applications, the results are satisfactory, are described below.