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电化学阻抗谱是以小振幅的正弦波电位(或电流)为扰动信号的电化学分析方法。它是研究电极表面生物敏感膜的形成、电极过程动力学以及测定固体电解质电导率的重要工具。以适体为新一代生物识别分子所构成的适体传感器,被用于蛋白质、药物和核酸的检测。电化学阻抗谱是表征和检测适体传感器识别前后电极界面性质变化的一种较新且强有力的分析技术,可以定量测定配体及实时研究适体与配体结合过程的动力学特性。本文在简要介绍电化学阻抗谱基本原理和分类的基础上,详细综述了近三年来电化学阻抗谱在适体传感器方面的研究应用。
Electrochemical impedance spectroscopy is a small amplitude sine wave potential (or current) for the disturbance signal electrochemical analysis. It is an important tool for studying the formation of biosensitive membranes on electrode surfaces, the electrode process kinetics, and the determination of the conductivity of solid electrolytes. Aptamer sensors, which are aptamers of the next generation of biomolecules, are used for the detection of proteins, drugs and nucleic acids. Electrochemical impedance spectroscopy (FTIR) is a new and powerful analytical technique for characterization and detection of changes in the interface properties of aptamer sensor before and after its identification. It can be used to quantitatively measure ligands and to study the kinetics of aptamer-ligand binding process in real time. Based on the brief introduction of the basic principles and classification of electrochemical impedance spectroscopy, the application of electrochemical impedance spectroscopy in aptamer sensor has been reviewed in detail in the last three years.