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为了研究缺血再灌注对骨骨各肌微血管和微循环的影响，采用气囊止血带，在常温下使兔后肢缺血２小时（ｎ＝８）和５小时（ｎ＝８）。在解除止血带后，通过活体显微镜观察再灌注最初１小时期间足背肌腱表面微循环变化，并于再灌注后１小时和７２小时，自胫前肌切取电镜和光镜标本，观察骨骨各肌微血管超微结构和组织形态变化。表现：①肢体缺血２小时再灌注后，虽然少数细静脉内可见轻度红细胞聚集和白细胞贴壁数增加，但腱外膜微循环可迅速恢复，分布也较均匀；骨骨各肌微血管仅有轻微损伤，肌纤维可存活。②肢体缺血５小时再灌注后，腱外膜微循环不能迅速而均匀地恢复，大部分区域发生无复流，细静脉内有大量白细胞粘附和聚集；骨骨各肌微血管内皮细胞显著肿胀，基底膜断裂，间质高度水肿，最终大部分肌纤维坏死。结果证明：缺血再灌注可导致微血管损伤和微循环紊乱，其严重程度取决于缺血时间的长短，并是决定缺血组织实质细胞转归的重要因素之一。
In order to investigate the effect of ischemia-reperfusion on the microvessels and microcirculation of the skeletal muscles, the hind limbs of rabbits were ischemia 2 hours (n = 8) and 5 hours (n = 8) at room temperature using balloon tourniquet. After the tourniquet was released, the changes of microcirculation on the tendon surface of dorsal flexor tendon during the first hour of reperfusion were observed by living microscope. Electron microscopy and light microscopy specimens were taken from the tibialis anterior muscle at 1 hour and 72 hours after reperfusion, Microvascular ultrastructure and tissue morphological changes. The results showed: (1) After 2 hours of ischemia, reperfusion of microvessels of tendon showed that the microcirculation of tendon outer membrane could be quickly recovered and distributed evenly, although the number of mild erythrocytes and leukocyte adherent cells increased in a few small veins. Slight damage, muscle fiber can survive. (2) limb ischemia 5 hours after reperfusion, tendon adventitia microcirculation can not be quickly and evenly recovered, no reflow occurred in most areas, a large number of white blood cells within the vein adhesion and aggregation; significant changes in bone microvascular endothelial cells , Basement membrane rupture, interstitial edema, eventually most of the muscle fiber necrosis. The results show that: ischemia-reperfusion can lead to microvascular injury and microcirculation disorder, the severity depends on the length of the ischemic time, and is an important factor in determining the outcome of ischemic parenchymal cells.