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目的 观察特异性单克隆抗体与非特异性多克隆抗血清荧光免疫偏振法测定肾移植病人全血环孢素浓度的差异及肝功能对血药浓度的影响。方法 对36位肾移植术后病人的84份环孢素(CsA)血样同时用特异性单克隆(MAFPIA)和非特异性多克隆抗体(PAFPIA)荧光免疫偏振分析法进行测定。对两种方法测得的数值进行统计学处理。结果MAFPIA与PAFPIA之间有较好的线性关系(相关系数为0.8947),两者的测得值具统计学显著性差异。肝功能异常的患者PAFPIA测得值显著大于MAFPIA测得值。与肝功能正常患者相比有明显差异,且两者的回归曲线参数均有明显不同。结论 对CsA血药浓度测定应采用特异性高的方法。
Objective To observe the differences of cyclosporine concentration in whole blood of patients with renal allografts and the effect of liver function on serum concentration of monoclonal antibody and non-specific polyclonal antiserum fluorescence immunopolarization assay. Methods 84 cyclosporin (CsA) blood samples from 36 renal transplant recipients were assayed by both fluorescent immunopolarization analysis with specific monoclonal antibody (MAFPIA) and non-specific polyclonal antibody (PAFPIA). The two methods measured the value of statistical processing. Results There was a good linear relationship between MAFPIA and PAFPIA (correlation coefficient: 0.8947). There was a statistically significant difference between the two. PAFPIA measured in patients with abnormal liver function was significantly higher than that measured with MAFPIA. Compared with patients with normal liver function, there are obvious differences, and the two regression curve parameters are significantly different. Conclusion CsA blood concentration should be determined by high specificity method.