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在pH60HAc－NH4Ac缓冲介质中，CTMAB存在下，1－（4-安替比林偶氮）－4氨基一2一萘甲酸（AANA）与铜生成2：1红色络合物，λmax＝540nm，ε=4.06×104L·Lmol-1·cm-1，铜含量在0－30μg/25内符合比尔定律，方法用于食品中铜的测定，结果满意。
In a pH 60 HAc-NH4Ac buffer medium, 1- (4-antipyrine azo) -4 amino-2-naphthoic acid (AANA) forms a 2: 1 red complex with copper in the presence of CTMAB, ε = 4.06 × 104L · mol-1 · cm-1, Beer’s law was obeyed in the range of 0-30μg / 25 and the method was applied to the determination of copper in food with satisfactory results.