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目的:利用铜螯合磁珠(MB-IMACCu)和基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术,对比分析难治性贫血伴幼稚细胞增多型(RAEB)骨髓增生异常综合征(MDS)患者血清蛋白质谱特征,寻找差异表达蛋白,构建RAEB型MDS辅助诊断的血清蛋白质组学模型。方法:根据性别及年龄,配比收集RAEB型MDS、急性髓性白血病(AML)和正常人的血清蛋白标本各10例,利用铜螯合磁珠进行蛋白分离,通过MALDI-TOF-MS测定标本的蛋白质谱图,对比分析差异蛋白峰,并构建诊断模型。结果:与正常对照比较,在RAEB患者血清发现47个异常表达峰(P<0.05),其中高表达峰16个,低表达峰31个;以AML患者为疾病对照,获得33个异常表达峰(P<0.05),在RAEB中呈低表达的12个,呈高表达的21个。以基因遗传法构建诊断模型,结果提示,模型的识别准确率均达100%,敏感性达89.42%。结论:MB-IMACCu和MALDI-TOF-MS技术平台有助于RAEB型MDS相关的血清异常表达蛋白的研究,并且能够有效地区分RAEB患者、AML患者和正常人;根据血清差异蛋白质的组合峰所构建的诊断模型可辅助RAEB型MDS的诊断。
OBJECTIVE: To compare and analyze the association between refractory anemia and immature cell polymyosis (RAEB) myelodysplastic syndromes using MB-IMACCu and matrix-assisted laser desorption / ionization time of flight mass spectrometry (MALDI-TOF- (MDS) in patients with serum protein profiles, looking for differentially expressed proteins, the construction of RAEB MDS assisted diagnosis of serum proteomics model. Methods: According to sex and age, RAEB-type MDS, acute myelogenous leukemia (AML) and normal serum samples were collected respectively in 10 cases. Copper chelate magnetic beads were used for protein separation. MALDI-TOF- Protein profiles, comparative analysis of differential protein peaks, and to build a diagnostic model. RESULTS: Compared with the normal controls, 47 abnormal expression peaks (P <0.05) were found in serum of RAEB patients, of which 16 were highly expressed and 31 were low expressed. In the patients with AML, 33 abnormal expression peaks P <0.05), 12 of which were low expression in RAEB and 21 were highly expressed. The genetic model to build a diagnostic model, the results suggest that the model identification accuracy of 100%, the sensitivity of 89.42%. CONCLUSION: The MB-IMACCu and MALDI-TOF-MS technology platforms contribute to the study of serum-associated proteins associated with RAEB-type MDS and can effectively discriminate between RAEB patients, AML patients and normal subjects. Based on the combination of serum differential proteins The constructed diagnostic model can aid in the diagnosis of RAEB-type MDS.