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Two novel V-shaped symmetric chromophores: E-2,8-bis(4-vinyl-4-carbazol-9-yl)dibenzothiophene (abbreviated as SK-G1) and E-2,8-bis(4-vinyl-4-triphenylamino) dibenzothiophene(abbreviated as ST-G1) have been synthesized and characterized. Their two photon absorption properties were measured by the open-aperture femtosecond Z-scan technique and the nanosecond nonlinear optical transmission (NLT), respectively, when pumped by Ti: sapphire laser at 750 nm and 800 nm.