论文部分内容阅读
本文作者建立了用反相高效液相色谱法测定红细胞膜中α-生育酚的方法。分离柱采用ODSC18柱,流动相为甲醇,采用荧光分光光度计为检测器.结果表明,α-生育酚在0.5×106~5.0×10─6浓度范围内有良好的线性关系.α-生育酚的回收率为96.2%,波动系数为9.66%。
The authors established a method for the determination of α-tocopherol in erythrocyte membranes by reversed-phase high performance liquid chromatography. Separation column using ODSC18 column, the mobile phase of methanol, using a fluorescence spectrophotometer as a detector. The results showed that α-tocopherol had good linearity in the concentration range of 0.5 × 106 ~ 5.0 × 10-6. The recovery of α-tocopherol was 96.2% with a coefficient of variation of 9.66%.