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青果(Canarium album Raeusch)经去果核,脱脂,酸水解,水解液回收溶剂后碱萃取,萃取液酸化,再以溶剂提取,回收溶剂,经柱层析,洗脱,重结晶,得成品,得率0.5％。经测试,结晶mp=239℃,M=170,经~1HNMR、~(13)CNMR、IR、MS检测,鉴定为没食子酸,理化性质与标准品或图谱基本一致,用ELISA法检测,抗HBsAg/HBeAg均有效,其效果与没食子酸对照品基本相同,认为没食子酸是青果中抗乙肝病毒有效成分之一。
Canarium album Raeusch is denucleated, defatted, acid-hydrolyzed, the solvent is recovered in the hydrolyzate, the alkali is extracted, the extract is acidified, the solvent is then extracted, the solvent is recovered, column chromatography, elution, recrystallization, and the finished product are obtained. Yield 0.5%. After testing, the crystals were mp=239°C, M=170, identified by ~1HNMR, ~(13)CNMR, IR, and MS, and identified as gallic acid. The physicochemical properties were basically the same as those of the standard or the spectrum. The anti-HBsAg was detected by ELISA. /HBeAg is effective, its effect is basically the same as the reference substance of gallic acid, and gallic acid is considered to be one of the effective components of anti-hepatitis B virus in the fruit.