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目的 :研究 Pin1(肽酰脯胺酰顺反式易构酶 )在髓系、淋系 10种恶性血液病细胞株中的表达情况以及其与不同细胞周期的关系。方法 :利用 Realtime- PCR检测血液病细胞株的 Pin1表达 ,同时用 Thymidine和 Nocoda-zole将细胞阻断在不同的细胞周期 ,用 Realtime- PCR及 Western Blot的方法 ,检测 Pin1的 m RNA和蛋白的表达水平。结果 :Pin1在恶性血液病细胞株中 m RNA的表达明显高于正常人骨髓单个核细胞的表达 (F=10 .5 0 8,P<0 .0 1) ,其在髓系血液病细胞株和淋系恶性血液病细胞株中的表达均较正常人骨髓单个核细胞明显增高 (分别为 F= 13.5 84 ,P<0 .0 1;F=5 .339,P<0 .0 5 )。 Pin1在 G1期 m RNA的表达水平显著高于 S期 (F=4 0 .5 72 ,P<0 .0 5 ) ,S期与 M期比较没有明显差异 (F=8.30 9,P>0 .0 5 )。但在蛋白水平上来看 Pin1的变化不明显。结论 :Pin1的 m R-NA在恶性血液病细胞中呈高表达 ,其表达水平与细胞周期有关 ,其中以 G1期为最高 ,S期最低。
Objective: To investigate the expression of Pin1 (peptidyl-aminocaproate transmutase) in 10 myeloid and lymphoid malignant hematological malignant cell lines and its relationship with different cell cycle. Methods: The Pin1 expression in hematological cell lines was detected by Real-time PCR. At the same time, cells were blocked by Thymidine and Nocoda-zole in different cell cycle. Real-time PCR and Western Blot were used to detect Pin1 mRNA and protein The expression level. Results: The mRNA expression of Pin1 in malignant hematological malignant cells was significantly higher than that in normal human bone marrow mononuclear cells (F = 10.500, P <0.01) And lymphoid malignant hematological cell lines were significantly higher than those in normal human bone marrow mononuclear cells (F = 13.584, P <0.01; F = 5.339, P <0.05). The expression level of Pin1 mRNA in G1 phase was significantly higher than that in S phase (F = 40.52, P <0.05). There was no significant difference between Pin 1 and M phase (F = 8.30 9, P> 0). 0 5). However, the change of Pin1 is not obvious at protein level. CONCLUSION: The m R-NA of Pin1 is highly expressed in hematological malignant cells, and its expression level is related to the cell cycle. Among them, the G1 phase is the highest and the S phase is the lowest.