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:以玉米杂交种郑 2 2×综 3的幼胚愈伤组织为材料 ,在加有NaCl和羟基脯氨酸 (Hyp)的筛选培养基上 ,连续筛选 5代 ,获得耐NaCl和耐Hyp的愈伤组织变异体 .研究结果表明 :(1)两种变异体具有较高的脯氨酸含量和较低的渗透势 ;(2 )在相同盐浓度的培养基上 ,脯氨酸含量和K+,Na+含量均为影响耐盐变异体耐盐性的影响因子 ,经比较排序为Hyp变异体 >耐NaCl变异体 >对照 ,耐Hyp变异体表现了最强的耐盐性 ;(3)耐Hyp变异体的高脯氨酸含量特性比耐NaCl变异体稳定 .用Hyp筛选耐盐变异体的的方法较为有效
: The immature embryos callus of Zheng 2 2 × Om 3 was used as the material and continuously selected for 5 generations on the screening medium supplemented with NaCl and hydroxyproline (Hyp) for NaCl and Hyp resistance The results showed that: (1) Both variants had higher proline content and lower osmotic potential; (2) At the same salt concentration, proline content and K + , Na + content were all the influencing factors of salt-tolerance of salt-tolerant mutants. Hyp variant> NaCl-tolerant variant> The high proline content of the mutant is more stable than that of the NaCl tolerant mutant. The method of screening salt tolerant variants with Hyp is more effective