论文部分内容阅读
目的:为研究锌转运体3(ZnT3)在神经系统功能中的作用提供形态学依据。方法:制备带有地高辛标记物的ZnT3反义RNA探针,取雄性SD大鼠脑、脊髓和脊神经节行冷冻切片,作ZnT3 mRNA原位杂交组织化学显色,杂交后用AKP标记的抗地高辛抗体显色。结果:脑内ZnT3 mRNA主要分布于海马结构,包括齿状回颗粒细胞和CA1～CA4区的锥体细胞,在梨状皮质、扣带后皮质中也有分布,显示的细胞较小;脑干中也有ZnT3 mRNA分布,面神经核处分布较多,网状结构中有少量分布;脊髓灰质前角内有少数胞体较大的细胞呈ZnT3 mRNA阳性;脊神经节内的多数神经元呈ZnT3 mRNA强阳性。结论:ZnT3 mRNA主要分布于海马、大脑皮质、杏仁核、脑干网状结构等可塑性较强结构和周围神经神经元中,提示ZnT3可能在学习、记忆、初级感觉传入和肌运动调控等方面发挥重要作用。
OBJECTIVE: To provide morphological evidence for studying the role of zinc transporter 3 (ZnT3) in nervous system function. METHODS: ZnT3 antisense RNA probes with digoxigenin labeling were prepared. The brain, spinal cord and dorsal root ganglion of male Sprague-Dawley rats were frozen and sectioned for ZnT3 mRNA in situ hybridization staining. After hybridization, they were labeled with AKP Anti-digoxin antibody color. Results: ZnT3 mRNA mainly distributed in hippocampus, including dentate gyrus granule cells and pyramidal neurons in CA1 ~ CA4 region. There was also distribution in piriform cortex and cingulate cortex, showing small cells in the brain stem There were also ZnT3 mRNA distributions, more distributions in the facial nucleus, and a few distributions in the reticular structure. In the anterior horn of the gray matter, a few cells with larger cell bodies were positive for ZnT3 mRNA. Most neurons in the spinal ganglia were strongly positive for ZnT3 mRNA. CONCLUSION: ZnT3 mRNA is mainly distributed in the plasticity structures such as hippocampus, cerebral cortex, amygdala, reticular structure of brain stem and peripheral nerve neurons, indicating that ZnT3 may play an important role in learning, memory, primary afferent affection and muscle movement regulation Play an important role.