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以西芹鲜根为试材,经乙醇浸提后,采用注层析法、化感作用检测法和GC-MS检测法,研究了西芹鲜根乙醇浸提液经第5、6次层析分离获得各层析流分与黄瓜枯萎病菌共培养,并计算出化感效果筛选出最佳流分,最后通过GC-MS检测出6次醇层物最佳流分中的化感物质成分,从而达到西芹鲜根中化感物质成分的纯化目的。结果表明:各流分均对黄瓜枯萎病菌具有较强抑制作用,其化感效果与乙醇对照差异显著,第5次层析最佳流分为RE211018、RE29554、RE62359和RE68656,化感效果(相对于乙醇对照)依次为63.05%、70.84%、69.38%和59.16%;第6次层析最佳流分为RE2110186、RE295548、RE623597和RE686568,其化感效果(同上)分别提高至70.14%、71.38%、64.84%和60.63%。西芹鲜根第6次醇层物4个最佳流分中含有酚类、酯类、胺类和烷烃类共4类11种化感物质,分别为2,4-二叔丁基苯酚、8,11-十八碳二烯酸甲酯、芥酸乙酯、邻苯二甲酸二甲酯、邻苯二甲酸异丁酯、邻苯二甲酸丁基2-乙基己基酯、邻苯二甲酸丁基酯2-戊基酯、邻苯二甲酸二丁酯、(Z)-9-十八烯酸酰胺、二十七烷和3-乙基-5-(2-乙基丁基)十八烷。
Fresh root of parsley was used as test material, after ethanol extraction, the method of injection chromatography, allelopathic effect and GC-MS were used to study the effects of the ethanol extract of celery root on the 5th and 6th chromatographies The chromatographic fractions were separated from the Fusarium oxysporum f.sp. cucumerinum and the optimal flotation index was calculated. Finally, the allelochemicals were detected by GC-MS, So as to achieve the purpose of purifying allelochemicals in fresh roots of celery. The results showed that all the fractions had a strong inhibitory effect on Fusarium oxysporum f. Cucumbers, and their allelopathic effects were significantly different from that of the ethanol control. The optimal flow of the fifth chromatography was RE211018, RE29554, RE62359 and RE68656. The allelopathic effects The results showed that the best flow of the sixth chromatography was RE2110186, RE295548, RE623597 and RE686568, the allelopathic effects were increased to 70.14% and 71.38%, respectively %, 64.84% and 60.63% respectively. The results showed that there are 11 kinds of allelochemicals in 4 kinds, including 2,4-di-tert-butylphenol, phenols, esters, amines and alkanes. Octadecadienoate, ethyl erucate, dimethyl phthalate, isobutyl phthalate, butyl 2-ethylhexyl phthalate, o- Pentyl formate, dibutyl phthalate, (Z) -9-octadecenoic acid amide, heptacosane and 3-ethyl-5- (2- ethylbutyl) Octadecane.