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目的：研究槲皮素（Quercetin，QUE）对培养人视网膜色素上皮（retinalpigmentepithelium，RPE）细胞增殖周期的影响。方法：用流式细胞仪测定细胞周期，观察不同浓度（200、100、50、1μmol／L）的QUE作用于RPE细胞48小时；以及QUE200μmol／L在不同作用时间（24～120小时），分别单独或与EGF共同对RPE细胞周期的影响；QUE连续作用于BPE细胞120小时后洗脱QUE，继续培养RPE细胞120小时内的细胞周期变化；QUE对RPE的细胞毒作用。结果：随QUE浓度增大，RPE细胞的S％减小；200μmol／LQUE在120小时内持续抑制RPE细胞进入S期；QUE对EGF促进RPE细胞进入S期的抑制效应更强；所有QUE作用组的细胞活力均在85％以上，移去QUE后，RPE细胞能重新进入细胞周期继续增殖。结论：QUE抑制RPE细胞从GO／GI期进入S期，具剂量依赖效应，在120小时内QUE能持续有效抑制RPE细胞进入S期，抑制效应具一定的可逆性，QUE对RPE细胞无细胞毒作用。
Objective: To study the effect of quercetin (QUE) on the cell cycle of cultured human retinal pigment epithelium (RPE). Methods: The cell cycle was measured by flow cytometry. The QUE of different concentrations (200, 100, 50 and 1μmol / L) were observed in RPE cells for 48 hours. The QUE200μmol / L was administered for 24 hours to 120 hours The effect of QUE alone or in combination with EGF on the cell cycle of RPE was observed. QUE was continuously eluted after 120 hours in BPE cells, and the cell cycle changes within 120 hours of RPE cells were continued. The cytotoxic effect of QUE on RPE cells. Results: With the increase of QUE concentration, S% of RPE cells decreased; 200μmol / LQUE continued to inhibit RPE cells entering S phase within 120 hours; QUE had stronger inhibitory effect on EGF promoting RPE cells entering S phase; Of cell viability were above 85%, after removal of QUE, RPE cells can re-enter the cell cycle to continue to proliferate. CONCLUSION: QUE inhibits the RPE cells from entering the S phase during the GO / GI phase in a dose-dependent manner. QUE can effectively and effectively inhibit RPE cells entering the S phase within 120 hours, with a certain reversibility of the inhibitory effect. QUE has no cytotoxic effect on RPE cells effect.