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利用克隆系ＮＧ１０８－１５细胞在膜片箝全细胞（ｗｈｏｌｅ－ｃｅｌｌｃｌａｍｐｒｅｃｏｒｄｉｎｇ）记录下，观察了国产ＴＴＸ单克隆抗体８Ａ５拮抗ＴＴＸ对跨膜钠电流的作用。结果表明，８Ａ５有效地抑制ＴＴＸ的作用：（１）在标准灌流液中加入１μｍｏｌ／ＬＴＴＸ，Ｎａ＋流立即被完全阻断，复加１μｍｏｌ／Ｌ８Ａ５于灌流液，可使Ｎａ＋流迅速恢复至对照水平；（２）若先以含１μｍｏｌ／Ｌ８Ａ５的溶液灌流细胞，再更置为含１μｍｏｌ／ＬＴＴＸ的标准液，Ｎａ＋流只有部分被抑制。
Using the clonal NG108-15 cells in whole-cell clamp recording, the effect of TTX monoclonal antibody 8A5 antagonizing TTX on transmembrane sodium current was observed. The results showed that 8A5 effectively inhibited the action of TTX: (1) 1μmol / LTTX was added into the standard perfusate solution, and the Na + flux was blocked immediately. The addition of 1μmol / L 8A5 to the perfusate rapidly restored the Na + flux to the control level ; (2) If the cells were perfused with a solution containing 1μmol / L 8A5 and then replaced with a standard solution containing 1μmol / L of LTT, only part of the Na + flow was inhibited.