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目的观察双歧杆菌(Bf)活制剂对大鼠溃疡性结肠炎(UC)肠黏膜上皮细胞(IECs)TLR2、TLR4、NF-κB基因表达的影响。方法 60只SPF级SD大鼠随机分3组:空白对照组、模型组、Bf组。模型组和Bf组大鼠建立TNBS诱导的UC模型。造模成功后,Bf组予双歧杆菌活菌制剂灌服,空白对照组及模型组不予任何处理。3周后处死全部大鼠,观察一般情况的变化,分离、提取IECs中的总RNA,RT-PCR法检测TLR2、TLR4的表达,免疫组化法检测TLR4、NF-κB表达。结果①Bf组症状、组织损害均较模型组明显减轻。②模型组TLR2、TLR4、NF-κB表达显著高于Bf组与空白对照组(P<0.01)。结论 UC组大鼠结肠上皮细胞TLR2、TLR4及NF-κB基因高表达,Bf组表达明显降低,推测Bf可通过抑制大鼠肠黏膜细胞TLR2、TLR4、NF-κB的表达而缓解肠道的炎症反应。
Objective To observe the effects of Bf live agents on the expression of TLR2, TLR4 and NF-κB in rat ulcerative colitis (UC) intestinal mucosa epithelial cells (IECs). Methods Sixty SPF SD rats were randomly divided into three groups: blank control group, model group and Bf group. TNBS-induced UC model was established in model group and Bf group. After the model was successfully established, the Bf group was fed with the viable Bifidobacterium preparations without any treatment in the blank control group and the model group. After 3 weeks, all the rats were sacrificed and the general changes were observed. The total RNA was extracted and extracted from IECs. The expressions of TLR2 and TLR4 were detected by RT-PCR and the expressions of TLR4 and NF-κB were detected by immunohistochemistry. Results ①Bf group symptoms and tissue damage were significantly reduced compared with model group. ② The expression of TLR2, TLR4 and NF-κB in model group was significantly higher than that in Bf group and blank control group (P <0.01). Conclusions The expression of TLR2, TLR4 and NF-κB in colonic epithelial cells of UC rats is high and the expression of Bf is significantly decreased in UC rats. It is speculated that Bf can relieve intestinal inflammation by inhibiting the expression of TLR2, TLR4 and NF-κB in intestinal mucosa of rats reaction.