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目的 探讨宫颈癌与人类组织相容性抗原 (HLA)表达缺失和人乳头瘤病毒 (HPV) 16、18感染的关系。方法 采用免疫组织化学S -P法检测特异性鼠抗人HLAⅠ类分子单克隆抗体及HPV16、18E6蛋白在宫颈癌组织中表达情况。结果 6 2例宫颈癌组织中有 4 2例HLAⅠ抗原呈阳性表达 ,2 0例呈阴性表达 ,阴性表达率 32 .3%。有 4 4例HPV16、18E6呈阳性表达 ,阳性率 70 .9% (4 4 / 6 2 )。在HPV16、18E6阳性表达组织中其HLAⅠ抗原缺失明显增加。HLAⅠ抗原阳性表达组织中CD3+ T细胞和CD8+ T细胞数量明显多于HLAⅠ抗原阴性表达组织 ,肿瘤恶性程度及淋巴结转移与HLAⅠ抗原下调有关。结论 宫颈癌组织存在HLAⅠ抗原部分缺失与HPV16、18感染密切相关。HLAⅠ抗原部分缺失 ,形成了免疫逃逸导致了肿瘤细胞的发生、发展、浸润、转移。
Objective To investigate the relationship between the loss of cervical cancer and human tissue-compatible antigen (HLA) expression and human papillomavirus (HPV) 16,18 infection. Methods Immunohistochemical S-P method was used to detect the expression of specific anti-human HLA class I monoclonal antibody and HPV16,18E6 protein in cervical cancer. Results 42 cases of cervical cancer tissues showed positive HLA Ⅰ antigen expression, 20 cases were negative expression, the negative expression rate of 32.3%. There were 44 cases of HPV16, 18E6 positive expression, the positive rate was 70.9% (44/62). In HPV16, 18E6 positive expression of its HLA I antigen deletion was significantly increased. The number of CD3 + T cells and CD8 + T cells in HLA Ⅰ antigen positive tissues was significantly higher than that in HLA Ⅰ antigen negative tissues. The degree of malignancy and lymph node metastasis were related to the downregulation of HLA Ⅰ antigen. Conclusion The partial deletion of HLA Ⅰ antigen in cervical cancer is closely related to HPV16 and 18 infection. Partial deletion of HLA I antigen, resulting in immune escape led to the occurrence of tumor cells, development, invasion, metastasis.