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目的:探讨新城疫病毒抑制人胃癌BGC-823细胞中人表皮生长因子受体2(human epidermal growth factor receptortype2,HER2)表达对胃癌细胞增殖的影响。方法:用反转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)以及Western印迹法研究新城疫病毒感染人胃癌细胞后HER2基因及其蛋白表达的变化,并采用噻唑蓝(thiazolylblue,MTT)试验观察新城疫病毒对胃癌细胞生长的抑制作用,流式细胞法(flow cytometry,FCM)法检测胃癌细胞的凋亡率。结果:新城疫病毒能抑制人胃癌细胞BGC-823中HER2基因及其蛋白质的表达,HER2基因表达下调使胃癌细胞生长受抑制,凋亡率增加(P<0.01)。结论:新城疫病毒杀伤胃癌细胞及诱导其凋亡的机制可能与抑制HER2基因及其表达有关.
Objective: To investigate the effect of Newcastle disease virus (NDV) on the proliferation of gastric cancer cell line BGC-823 in vitro and in vitro. Methods: The changes of HER2 gene and its protein expression in Newcastle disease virus infected human gastric cancer cells were studied by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Thiazolylblue (MTT) assay was used to observe the inhibitory effect of Newcastle disease virus on the growth of gastric cancer cells. Flow cytometry (FCM) was used to detect the apoptosis rate of gastric cancer cells. Results: Newcastle disease virus could inhibit the expression of HER2 gene and its protein in human gastric cancer cell line BGC-823. The down-regulation of HER2 gene inhibited the growth of gastric cancer cells and increased the apoptosis rate (P <0.01). Conclusion: The mechanism of Newcastle disease virus killing gastric cancer cells and inducing apoptosis may be related to the inhibition of HER2 gene and its expression.