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研究了低温、CaCl2 、PEG 3种预处理对 18个小麦品种成熟胚愈伤组织形成的影响 ,结果表明 :不同预处理对小麦成熟胚愈伤组织的形成有显著影响。PEG处理对小麦成熟胚愈伤组织的形成具有显著促进作用 ;低温和CaCl2 溶液处理对小麦成熟胚愈伤组织的影响和小麦品种关系密切 ,品种间差别较大。
The effects of low temperature, CaCl2 and PEG pretreatment on the callus formation of mature embryos of 18 wheat cultivars were studied. The results showed that different pretreatments had a significant effect on the formation of callus from mature embryos of wheat. PEG treatment significantly promoted the formation of callus from mature embryos in wheat. The effects of low temperature and CaCl2 treatment on the callus of wheat mature embryos were closely related to wheat cultivars, and the differences among cultivars were significant.