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利用JoinMap 4.0软件包,以德国镜鲤选育系为祖父母所培育的自交F2群体的68个个体为作图群体,首次以新型分子标记单核苷酸多态性(Single Nucleotide Polymorphism,SNP)为主要作图标记,以微卫星(Simple Sequence Repeats,SSR)和表达序列标签(Expressed Sequence Tag,EST)为辅助标记,采用CP(CrossPollinators)模型构建鲤的遗传连锁图谱。构建的遗传连锁图谱含有560个标记(174个SSR标记、41个EST-SSR标记和345个SNP标记),分布在50个连锁群上,最大连锁群由60个标记组成,最小连锁群仅含2个标记,平均每个连锁群有11.2个标记。最大连锁群的图距为198.1厘摩(centimorgan,cM),最小的连锁群图距为1.5 cM,图谱总图距为3 295.92 cM,标记间平均间距为7.21 cM,图谱覆盖率为76.26%。构建的图谱为中等密度的遗传连锁图谱可为进一步的鲤相关经济性状的QTL定位研究和分子标记辅助选择育种(MAS)打下基础。研究亮点:首次以新型分子标记SNP为主要作图标记并以SSR标记为辅助标记构建鲤遗传连锁图谱;有来自表达基因编码区的41个EST-SSR标记分布在图谱上;构建的图谱标记类型丰富,数量大,密度高,为进一步的鲤重要经济性状的QTL定位和分子标记辅助育种打下基础。
Using JoinMap 4.0 software package, 68 individuals of selfing F2 population that were cultivated by the German mirror carp breeding system as the grandparents were used as mapping populations. For the first time, a new single nucleotide polymorphism (SNP) The main mapping markers were Simple Sequence Repeats (SSR) and Expressed Sequence Tags (ESTs). The genetic linkage maps of common carp were constructed by CP (CrossPollinators) model. The constructed genetic linkage map contained 560 markers (174 SSR markers, 41 EST-SSR markers and 345 SNP markers) distributed on 50 linkage groups, the largest linkage group consisted of 60 markers, and the smallest linkage group contained only 2 markers, with an average of 11.2 markers per linkage group. The largest linkage group was 198.1 centimeter (cM), the smallest linkage group was 1.5 cM, the total length of the map was 3 295.92 cM, the average distance between markers was 7.21 cM, and the coverage rate of the map was 76.26%. The constructed genetic linkage map with medium density could lay a foundation for further QTL mapping and molecular marker-assisted selection breeding (MAS) of common carp related economic traits. Research highlights: For the first time, a novel molecular marker SNP was used as the main mapping marker and the SSR marker as the auxiliary marker to construct the genetic linkage map of common carp. 41 EST-SSR markers from the coding region of the expressed gene were mapped on the map. Rich, large in number and high in density, laying a foundation for further QTL mapping and molecular marker-assisted breeding of important economic traits of common carp.