对氮蓝四唑（NBT）-光照法实验操作进行了改进，用改进后的光照法测定了2种自制的SOD模拟物Cu（Gly-Gly）H2O和Cu（Ser）2与超氧阴离于自由基O2-应的动力学参数，其值与其他方法所测一致．对改进效果进行了讨论和评价．结果表明，经改进的光照法在简化操作、提高效益及测量精度方面有很大改观． The experimental operation of NBT - light irradiation was improved. Two kinds of self - made SOD mimics Cu (Gly-Gly) H2O and Cu (Ser) 2 and superoxide anion The kinetic parameters of free radical O2- should be consistent with those measured by other methods. The improvement effect was discussed and evaluated. The results show that the improved lighting method greatly simplifies the operation, improve efficiency and measurement accuracy has been greatly improved.