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目的：研究９９ｍ Ｔｃｃｃ Ｍ ４嵌合抗体用于肺癌放射免疫诊断。方法：用直接法９９ｍ Ｔｃ 标记嵌合抗体 ｃｃ Ｍ ４对１７例疑肺癌患者行放射免疫显像（ Ｒ Ｉ Ｉ），同时用１２５Ⅰ标记 ｃｃ Ｍ ４检测患者血清中的 Ｔ Ａ Ｇ７２抗原水平。显像后２周检测病人血清中的人抗鼠抗体（ Ｈ Ａ Ｍ Ａ）水平。结果：肺癌患者中 Ｔ Ａ Ｇ７２抗原水平明显高于正常对照者（ Ｐ＜ ００５），阳性率为４６４％ 。注射９９ｍ Ｔｃｃｃ Ｍ ４后１２ ｈ 癌灶对心肝的靶／非靶比值分别为２６６和２０８，选取此时的显像结果与手术及病理对比，灵敏度为７２２％ （１３／１８），对于１４个原发灶 Ｒ Ｉ Ｉ示１１个有放射性浓集，灵敏度为７８６％ ，纵隔转移灶３个中２个 Ｒ Ｉ Ｉ示放射性浓集，１个肝转移灶阴性。３例非癌性病变未见放射性浓集。注射抗体２周后病人血清中 Ｈ Ａ Ｍ Ａ 均未见阳性反应。结论：９９ｍ Ｔｃｃｃ Ｍ ４肺癌 Ｒ Ｉ Ｉ诊断原发性肺癌是一种可行的方法。
Objective: To study 99m Tc cc M 4 chimeric antibody for radioimmunoassay of lung cancer. Methods: Radioimmunoimaging (R I I) was performed on 17 patients with suspected lung cancer using the direct method of 99m Tc-labeled chimeric antibody cc M 4, and the level of T A G72 antigen in serum was detected by 125Ⅰ-labeled cc M 4 . Human anti-mouse antibody (H A M A) levels in the patient’s sera were tested two weeks after imaging. Results: The levels of T A G72 antigen in lung cancer patients were significantly higher than those in normal controls (P <005), the positive rate was 464%. The target / non-target ratios of hepatocellular carcinoma to heart and liver at 12 h after 99m Tccc M 4 injection were 266 and 208, respectively. The imaging results at this time were compared with the results of surgery and pathology, and the sensitivity was 722% 13/18). For the 14 primary tumors, R I I showed 11 radioactive concentrations with a sensitivity of 78.6%. Two of the 3 mediastinal metastases showed radioactive enrichment and one liver metastasis negative. Three cases of non-cancerous lesions no radioactive concentration. Two weeks after the injection of the antibody, no positive reaction was found in the serum of the patients with H A M A. Conclusion: 99m Tc cc M 4 lung cancer R I I diagnosis of primary lung cancer is a viable method.