用ＰＣＲ扩增出ＨＧＶＥ２全基因，克隆进杆状病毒表达载体ｐＦＡＳＴＢＡＣＨＴａ中，构建成重组转座载体ｐＦＡＳＴＢＡＣＥ２，转化ＤＨ１０ＢＡＣ大肠杆菌感受态细胞，筛选阳性菌落，抽提大分子质粒ＤＮＡ，获得含ＨＧＶＥ２基因的重组杆状病毒穿梭载体，转染昆虫草地夜蛾Ｓｆ９细胞，出现细胞病变后，收集含有重组病毒颗粒的培养上清，重新感染草地夜蛾Ｓｆ９单层细胞及甜菜夜蛾幼虫，分别收集Ｓｆ９细胞和甜菜夜蛾幼虫体内的血淋巴细胞，进行１２％ＳＤＳ聚丙烯酰胺凝胶电泳，可见表达的融合蛋白带，经亲和层析进行蛋白纯化，用ＥＬＩＳＡ方法检测各类血清标本，初步研究ＨＧＶＥ２糖蛋白的抗原性 The whole HGVE2 gene was amplified by PCR and cloned into the baculovirus expression vector pFASTBACHTa to construct the recombinant transposase pFASTBACE2. The recombinant plasmid was transformed into DH10BAC E.coli competent cells, positive colonies were screened, and the plasmid DNA was extracted to obtain HGVE2 gene recombinant baculovirus shuttle vector transfected Sf9 insect cells, cytopathic effect, the collection of recombinant virus particles containing the culture supernatant, re-infected with Sf9 monolayer cells and beet armyworm larvae, respectively The blood lymphocytes of Sf9 cells and beet armyworm larvae were collected and subjected to 12% SDS-polyacrylamide gel electrophoresis. The expressed fusion protein bands were visualized by affinity chromatography, and various types of serum samples were detected by ELISA , A preliminary study of HGVE2 glycoprotein antigenicity