论文部分内容阅读
AIM:To determine the expression of estrogen receptor(ER)β in Chinese colorectal carcinoma (CRC) patients.METHODS:ERβ expression in CRC was investigated byimmunohistochemical staining of formalin-fixed,paraffin-embedded tissue sections from 40 CRCs,10 colonic adenomas,and 10 normal colon mucosa biopsies.The percentage ofpositive cells was recorded,mRNA expression of ERα andERβ in 12 CRC tissues and paired normal colon tissues weredetected by RT-PCR.RESULTS:Positive ER immunoreactivity was present in partof normal epithelium of biopsy (2/10),adenomas (3/10),and the sections of CRC tissue,most of them were nuclearpositive.In CRCs,nudear ERβ immunoreactivity was detectedin over 10% of the cancer cells in 57.5% of the cases andwas always associated with cytoplasmic immunoreactivity.There was no statistical significance between ERβ positiveand negative groups in regard to depth of invasion and nodalmetastases.Of the 12 CRC tissues and paired normal colontissues,the expression rate of ERα mRNA in CRC tissue andcorresponding normal colon tissue was 25% and 16.6%,respectively.ERβ mRNA was expressed in 83.3% CRC tissueand 91.7% paired normal colon tissue,respectively.Therewas no significant difference in ERβ mRNA level betweenCRC tissues and paired normal colon tissues.CONCLUSION:A large number of CRCs are positive forERβ,which can also be detected in normal colonic epithelia.There is a different localization of ERβ immunoreactivityamong normal colon mucosae,adenomas and CRCs.ERαand ERβ mRNA can be detected both in CRC tissue and incorresponding normal colon tissue.A post-transcriptionalmechanism may account for the decrease of ERβ proteinexpression in CRC tissues.
AIM: To determine the expression of estrogen receptor (ER) β in Chinese colorectal carcinoma (CRC) patients. METHODS: ERβ expression in CRC was investigated by immunohistochemical staining of formalin-fixed, paraffin- embedded tissue sections from 40 CRCs, 10 colonic adenomas, and 10 normal colon mucosa biopsies. The percentage ofpositive cells was recorded, mRNA expression of ERα andERβ in 12 CRC tissues and paired normal colon tissues weredetected by RT-PCR.RESULTS: Positive ER immunoreactivity was present in partof normal epithelium of biopsy (2 / 10), adenomas (3/10), and the sections of CRC tissue, most of them were nuclearpositive.In CRCs, nudear ERβ immunoreactivity was detected in over 10% of the cancer cells in 57.5% of the cases andwas always associated with cytoplasmic immunoreactivity . There was no statistical significance between ERβ positive and negative groups in regard to depth of invasion and nodal metastases. Of the 12 CRC tissues and paired normal colontissues, the expression rate of E Rα mRNA in CRC tissue and corresponding normal colon tissue was 25% and 16.6%, respectively. ERβ mRNA was expressed in 83.3% CRC tissue and 91.7% paired normal colon tissue, respectively. No significant difference in ERβ mRNA level between CRC tissues and paired normal colon tissues.CONCLUSION: A large number of CRCs are positive forERβ, which can also be detected in normal colonic epithelia. There is a different localization of ERβ immunoreactivityamong normal colon mucosae, adenomas and CRCs.ERαand ERβ mRNA can be detected both in CRC tissue and incorresponding normal colon tissue. A post-transcriptional mechanism of action may account for the decrease of ERβ proteinexpression in CRC tissues.