论文部分内容阅读
细胞学检查在临床肿瘤诊断中具有重要意义,近几年来细胞标本做免疫组织化学染色已广泛用于鉴别肿瘤细胞来源及良恶性.细胞标本取材方便,形态直观,但其缺点是不宜长期保存.若制成涂片后可长期保存但免疫细胞化学染色不理想,抗原活性保存不完全.为此,作者在组织配型淋巴细胞保存法的基础上改良而成一种细胞长期保存法,既适作于做免疫组织化学染色,又能完好的保存细胞形态,且保存时间可长达1.5年.方法简便实用,现介绍如下:1 方法1.1 各种渗液如胸水、腹水、尿液、肺泡灌洗液等装入离心试管(5ml),1500r/min离心,弃上清液后留其沉淀(气管镜涮检标本、骨髓等无须此步骤)1.2 标本离心后加入20%小牛血清1ml,20%二甲
Cytological examination is of great significance in the diagnosis of clinical tumors. In recent years, immunohistochemical staining of cell specimens has been widely used to identify the origin and benign and malignant tumor cells. Cell specimens are easy to draw and intuitive, but their shortcomings are not suitable for long-term preservation. If the smear can be preserved for a long period of time but the immunocytochemical staining is not ideal, the antigen activity is not completely preserved. For this reason, the authors improved the method of long-term preservation of cells on the basis of tissue-matched lymphocyte preservation method, which is suitable for both. For immunohistochemical staining, it can preserve the cell morphology intact, and the preservation time can be as long as 1.5 years. The method is simple and practical, is described as follows: 1 method 1.1 various exudates such as pleural effusion, ascites, urine, bronchoalveolar lavage The liquid and so on into a centrifuge tube (5ml), centrifuged at 1500r/min, abandoned the supernatant and leave the precipitate (observed by the bronchoscope specimen, bone marrow, etc.) 1.2 Centrifuge the sample and add 20% calf serum 1ml, 20% Dimethyl